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Summary of Research Projects (Supports in 2004
Fiscal Year)
| Subjects |
Study on the vascular development in Arabidopsis |
| Representative researcher |
School of Science,the University of Tokyo
Shinichirou SAWA |
| In most of dicotyledonous plants, vascular
tissues in the leaf present a reticulate venation pattern. We have isolated
and characterized an Arabidopsis mutant defective in vascular network defective
mutants, van3. van3 mutants showed discontinuous vascular pattern,
and the VAN3 encodes an ARF GAP protein regulating the membrane
trafficking in the trans Golgi network. To elucidate the molecular natures
controlling vein patterning process through membrane trafficking, we searched
VAN3-interacting proteins. by using a yeast two hybrid system. As a result,
we isolated the plant dynamin, DRP1A as a VAN3 interacting protein. Spatial
and temporal expression pattern by DRP1A::GUS and VAN3::GUS were similar
to each other. Subcellular localization of VAN3 is completely overlapped
to that of the DRP1A. drp1a showed disconnected vascular network,
and drp1a mutation enhanced the phenotype of vascular discontinuity
of the van3 mutant in the drp1a van3 double mutant. Furthermore,
the drp1 mutation enhanced discontinuous vascular pattern of the
gnom mutant, which is the same effect as that of van3 mutation.
These results indicated that DRP1 modulates the VAN3 function
in vesicle budding from TGN, regulating vascular formation in Arabidopsis. |
|
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