|
Summary of Research Projects (Supports in 2005
Fiscal Year)
| Subjects |
Function analysis of plant photoreceptor, phototropin
family |
Representative
researcher |
University of Tsukuba
Takatoshi KAGAWA
|
| In seed plants, chloroplast relocation
movement is mediated by a phototropin which is constructed with two LOV
domains with FMN and one kinase domain. In Arabidopsis, accumulation movement
is mediated by phot1 and phot2, and avoidance movement is mediated by phot2.
In lower plants such as green algae and fern, the chloroplast movement
should be controlled by chimeric photoreceptor of red-light absorbing photoreceptor
phytochrome and the phototropin as well as the phototropin. In this study,
we demonstrated about photoreceptors for the chloroplast movement. As results
of analysis of phot2-transgenic plants on the chloroplast avoidance in
Arabidopsis, the velocity should be dependent upon amount of phot2 but
leaf expansion is rescued by phot2 accumulation which is lower than threshold
level of immunoblotting. In photoreceptors of lower plants, we analyzed
photometrically photoreceptive domains which were expressed modified E.
coli. As the results, both phytochrome and LOV domains of phy3 works
as photoreceptive domain. However, phytochrome domain of green algae neochrome
works as photoperception but the LOV domain could not absorb blue light
because of absent of chromophore, FMN. |
|
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